Republican Collection of Microorganisms LLP carries out 7 grant projects
Name topic of the project: AP09260001 Obtaining a biological product for long-term storage providing high quality of fruits growing in Kazakhstan
The purpose of the grant project: to develop a biological product for processing the harvest of orchards in Kazakhstan, increasing the shelf life of fruits by inhibiting the growth of microbes that cause spoilage and decay.
Objectives of the grant project
- Isolate, select and identify antagonist strains (lactic acid bacteria, yeast fungi, etc.) from the surface of fruits that suppress the growth of microbes that cause post-harvest spoilage of fruits (Penicillium expansum – blue mold and Colletotrichum acutatum – rot) in the storehouses of garden farms in southern Kazakhstan
- Search for the most effective formula of a biological product on a model of provoked spoilage of various varieties of apples (3-4 varieties) in laboratory conditions:
2.1. Application of pure fermentate or supernatant of antagonist strains to treat apples;
2.2. The use of a diluted suspension of microbial cells of the antagonist strain for the treatment of various varieties of apples
2.3. The combined effect of a suspension of cells of the antagonist strain with treatment with dry warm air and / or sodium bicarbonate solution (baking soda)
- Carrying out research related to the study of the physicochemical properties of substances that provide the action of antagonist strains in suppressing microbes that cause damage to fruits
- Development of methods and methods for storing a biological product
- Preparation for field testing of a biological product:
5.1. Development of a biological product in a semi-industrial volume;
- 2. Development of the most effective technology for applying a biological product to fruits in orchards immediately after harvesting
6 Conducting field trials of a biological product in storage facilities of orchards in southern Kazakhstan
7 Based on the results of field trials of the biological product, technical regulations for experimental and industrial development will be developed to extend the shelf life and improve the quality of fruit products in the gardening economy of Kazakhstan
8 Processing of the harvest of garden farms with a biological product, according to the development implementation act
9 Evaluation of the development implementation efficiency
10 Submission of an application for registration of intellectual property for a developed biological product to the National Institute of Intellectual Property of the Republic of Kazakhstan
Expected project results:
- A collection of strains of microorganisms with a high degree of anatagonism to microbes that cause spoilage of fruits grown in the orchards of southern Kazakhstan will be collected, their genetic identification will be carried out.
- As a result of the analysis of experimental data, the most effective formula (s) and methods of applying a biological product will be found, which prolongs the shelf life of apples and other fruit crops.
- The physicochemical properties of the compounds providing the antibacterial action of antagonist strains against microbes that cause fruit spoilage will be studied.
- Methods and methods of biological product storage will be worked out.
- The required volume of biological product will be obtained and an effective technology of its application will be developed for field trials in the gardening economy of Kazakhstan.
- Field trials of the biological product will be carried out in the storage facilities of orchards in southern Kazakhstan.
- An act will be developed for the implementation of experimental and industrial development to extend the shelf life and improve the quality of fruit products in the gardening economy of Kazakhstan.
- Processing of the garden farm harvest with a biological product will be carried out.
Name topic of the project: AP14869952 Development of biopreparations to improve the productivity and sustainability of the development of young birds in poultry
The purpose of the grant project: the project is to create a probiotic biopreparation with specified properties of microorganisms used as starter feed for growing young birds.
Objectives of the grant project
- Screening cultures of microorganisms of the highest industrial value as probiotic feed additives.
- Study of cultural, biochemical and physiological properties of selected strains of microorganisms as probiotics.
- Study the antagonism of the most active bacterial cultures to opportunistic pathogens.
- Create a consortium of different cultures of microorganisms with high probiotic activity.
- Selection of a basic nutrient medium for cultivation of microorganisms and evaluation of probiotic potential of the created consortium.
- Working out and optimizing the parameters of deep cultivation in a laboratory fermenter.
- Study of the effectiveness of the use of a probiotic drug in laboratory conditions
- Development and optimization of parameters of deep cultivation in a semi-industrial fermenter
- Study of the effectiveness of the use of a probiotic drug in production conditions
Expected project results:
- Cultures of microorganisms of highest industrial value as probiotic feed additives will be screened.
- The cultural, biochemical and physiological properties of isolated strains of microorganisms selected as probiotics will be studied.
- The antagonism of the most active bacterial cultures to opportunistic pathogens will be examined.
- A consortium of different cultures of microorganisms with high probiotic activity will be created.
- The main nutrient media for cultivation of microorganisms will be selected and the probiotic potential of the created consortium will be evaluated.
- Parameters of deep culturing in laboratory and semi-industrial fermenter will be worked out and optimized.
- The effectiveness of application of the probiotic preparation under laboratory and industrial conditions will be studied.
- Based on the results of the study will be published:
- at least 3 (three) articles and/or reviews in peer-reviewed scientific journals indexed in the Science Citation Index Expanded database Web of Science and/or having a CiteScore percentile in the Scopus database of at least 50 (fifty);
- and at least one (1) article or review in a peer-reviewed foreign or domestic publication recommended by the SQAC;
- or at least two (2) articles and/or reviews in peer-reviewed scientific journals included in the first and/or second quartile of the Web of Science impact factor and/or having the CiteScore percentile in the Scopus database of at least 65 (sixty-five);
- or at least one (1) article or review in a peer-reviewed scientific publication that is in the first (1) quartile by impact factor in the Web of Science database and/or has a CiteScore percentile in the Scopus database of at least 80 (eighty).
Name topic of the project: AP19678630 Obtaining a broad-spectrum biological fungicide to ensure and maintain high yields of farms in Kazakhstan
The purpose of the grant project: to develop a preparation of a broad-spectrum biological fungicide based on a consortium of antagonist yeasts that ensures the production and maintenance of high yields of farms.
Objectives of the grant project
1-Isolate from the microbiota of the soil, rhizo- and phyllosphere of fruit and vegetable crops (tomatoes, grapes, cherries, peaches, apricots, apple trees) isolates with characteristic cultural and morphological features of yeast;
2-Screen yeast isolates for antagonistic activity against phytopathogenic fungi (Penicillium expansum – blue mold and Botrytis cinerea – gray mold) and select isolates with antagonistic activity exceeding the half-maximal value of activity (with a relative growth of the fungus less than 50%);
3-Screen the yeast isolates selected in task No. 2 for their growth tolerance to conditions of different temperatures (15–37 °C);
4- Screen the yeast isolates selected in task #3 for biofilm formation and select the isolates with the highest rates of biofilm formation activity;
5- Carry out the identification of yeast isolates selected in task 4;
6- Screen the yeast isolates from Task 5 for biofungicidal activity on selected affected fruits against B. cinerea, A. niger and R. stolonifer on table grapes and B. cinerea and R. stolonifer on tomatoes;
7- Screen the yeast isolates tested in task 6 for biofungicidal activity in in vivo experiments with bunches of table grapes against B. cinerea, A. niger and R. Stolonifer;
8- Create a consortium “KM” from the yeast isolates selected in Tasks 6 and 7 with the maximum biofungicidal activity;
9- To develop the biomass of the consortium “KM”;
10- Conduct field trials on table grapes in a commercial vineyard located in the Almaty region using the KM consortium and compare its action with the synthetic fungicide Topaz (Syngenta Crop Protection AG);
11- Based on the results of field trials of the biological product, develop a technological regulation for the biological product;
12- Submit an application for registration of intellectual property for the developed biological product to the National Institute of Intellectual Property of the Republic of Kazakhstan.
Expected project results:
Within the framework of this project, a biofungicide preparation will be developed to combat diseases of valuable varieties of fruit and vegetable crops. The basis of the biological product will be a consortium of antagonist yeast strains with the most effective parameters of biological plant protection, identified in experiments in vitro and in vivo. Antagonist yeasts will be isolated from the soil microbiota, roots, and phyllosphere of vegetable and fruit plants.
Name topic of the project: AP19679554 Development of a complex biotechnology of the use of probiotic microorganisms and medicinal plants
The purpose of the grant project: The goal is to develop a comprehensive biotechnology for the use of medicinal plants and probiotic microorganisms for the prevention and treatment of bacterial and parasitic infections in aquaculture.
Objectives of the grant project
- Isolation of pathogens (bacterial, fungal, parasitic (helminths) from infected aquaculture of fish farms and their identification;
- Study of biological properties and screening of lactic acid bacteria based on antimicrobial and antifungal activity.
- Collection of medicinal plants (rosemary/wormwood/stubble, garlic/motherwort/ ledum/celandine/ficus leaves/nettle and others) promising for combating infectious diseases in aquaculture (fish);
- Isolation of biologically active compounds, essential oils, extracts and tinctures from medicinal plants. Determination of the effect of lactic acid bacteria on bacterial pathogens and aquaculture (underyearlings of carp/trout/carp) in vitro.
- Selection of concentrations, dosages of the action of active substances of medicinal plants in relation to the survival of pathogens in vitro;
- Selection of the concentration of probiotics in the aquatic environment / in the feed formulation to combat infectious diseases of various etiologies;
- Evaluation of the effect of medicinal plants and probiotics on biometric growth indicators (weight gain and specific growth rate), activity, intestinal pH in underyearlings/carp/trout/carp. Histological and hematological analysis of experimental and control groups in vitro;
- Assessment of the influence of medicinal plants and probiotics on the quality of the aquatic environment. Determination and analysis of indicators of organic substances and oxygen in the aquatic environment;
- Determination of the antiviral activity of biologically active compounds obtained from medicinal plants. Study of the inhibition of aquatic pathogens of the aquatic environment by medicinal plants;
- Evaluation of the effect of medicinal plants and probiotics on biometric growth parameters (weight gain and specific growth rate), activity, intestinal pH in carp/trout/carp fingerlings in vitro / in vivo. Histological and hematological analysis of experimental and control groups;
- Development of an integrated biotechnology for the use of medicinal plants and probiotic microorganisms for the prevention and treatment of the development of various pathological pathogens in fish aquaculture (aeromonosis, opisthorchiasis, and others);
- Bionformatic analysis of the obtained data. Publication of articles and preparation of the final report.
Expected project results:
- Pathogens (bacterial, fungal, parasitic (helminths) from infected fish farm aquaculture will be isolated and identified.
- The biological properties will be studied and the screening of lactic acid bacteria based on antimicrobial and antifungal activity will be carried out.
- Medicinal plants promising for combating infectious diseases in aquaculture (fish) will be collected.
- Bioactive compounds/tinctures from medicinal plants will be isolated. The effect of lactic acid bacteria on bacterial pathogens and in vitro aquaculture will be determined.
- The concentration, dosage of the action of the active substances of medicinal plants in relation to the survival of pathogens in vitro will be selected;
- The concentration of probiotics in the aquatic environment / in the feed formulation will be selected to combat infectious diseases of various etiologies;
- The impact of medicinal plants and probiotics on biometric growth indicators (weight gain and specific growth rate), activity, intestinal pH in underyearlings/carp/trout/carp will be assessed. Histological and hematological in vitro analysis of experimental and control groups;
- An assessment will be made of the impact of medicinal plants and probiotics on indicators of the quality of the aquatic environment. Determination and analysis of indicators of organic substances and oxygen in the aquatic environment;
- The antiviral activity of biologically active compounds derived from medicinal plants will be determined;
- The influence of medicinal plants and probiotics on biometric growth parameters (weight gain and specific growth rate), activity, intestinal pH in fingerlings/carp/trout/carp in vitro/in vivo will be assessed. Histological and hematological analysis of experimental and control groups;
- An integrated biotechnology for the use of medicinal plants and probiotic microorganisms for the prevention and treatment of the development of various pathological pathogens in fish aquaculture will be developed;
- Boudin carried out a bio-informational analysis of the obtained data. Scientific articles will be published and a final report prepared.
- At least 1 (one) article or review will be published in a peer-reviewed scientific publication indexed in the Science Citation Index Expanded and included in the 1st (first) quartile by impact factor in the Web of Science database and (or) having a CiteScore percentile in the Scopus database of at least 80 (eighty);
- At least 1 (one) article or review will be published in a peer-reviewed foreign or domestic publication recommended by the CQASHE;
- At least 1 application for a patent of the Republic of Kazakhstan and at least 1 application to the Eurasian Patent Office will be filed.
Name topic of the project: AP19679863 Production of a probiotic preparation for therapeutic and prophylactic applications
The purpose of the grant project: Production of probiotic preparation for use in prevention and treatment purposes.
Objectives of the grant project
- Selection of production probiotic strains with molecular biological characteristics.
- Development of the optimal form of probiotic preparation.
- Obtaining an preproduction series of products.
- Evaluation of safety of probiotic using toxicity study.
- Study of a probiotic for therapeutic activity.
- Development of regulatory and technical documentation for the production of probiotics.
- Production of a probiotic preparation.
- Certification of final products for compliance with the requirements established by the Legislation of the Republic of Kazakhstan in the field of technical regulation.
- Preparation of an application for registration of intellectual property objects, publication of scientific papers.
Expected project results:
- Industrial strains with confirmed species identification and probiotic properties will be selected. Molecular genetics characterization of the probiotic strain will be carried out using whole genome sequencing.
- The optimal form of the probiotic preparation will be determined and the quality indicators for the corresponding dosage form will be evaluated.
- The technology of obtaining a biological product will be worked out and a pilot batch will be obtained in accordance with the applicable requirements for proper production to ensure safety, purity and stability.
- Toxicity study for evaluation of safety of the probiotic dosage form in laboratory animals will be tested.
- Therapeutic activity of probiotic dosage form on laboratory animals will be tested.
- Regulatory and technical documentation for the production of probiotics will be developed.
- A probiotic preparation will be produced.
- For further sale of finished products, the procedure of mandatory certification of products will be carried out in order to confirm the conformity of products with the mandatory requirements of legislation in the field of technical regulation.
- The final products will be certified for compliance with the requirements of legislation in the field of technical regulation.
Name topic of the project: AP19678528 Obtaining of biopreparations that provide growth stimulation and protection of planting material in Kazakhstan’s forest parks and horticultural farms
The purpose of the grant project: The purpose of the project: To develop and obtain biological preparations that provide growth stimulation, protection and survival of seedlings of ornamental and fruit crops in forest park areas and horticultural farms in Kazakhstan.
Objectives of the grant project
- Collection and study of cultivated microbiota of the rhizosphere of halophyte steppe plants of Kazakhstan: white quinoa (Atriplex cana), Schrenk’s wormwood (Artemisia schrenkiana), tamarix (Tamarix), saxaul (Haloxylon), dzhuzgun (Calligonum), and others, growing in Almaty, Turkestan, Akmola, regions, known for their high adaptive properties:
1.1 Collection of soil samples from the rhizosphere of halophyte steppe plants (at least 100 samples);
1.2 Isolation of cultivated microorganisms from soil samples;
1.3 Identification of microorganisms sequencing of 16S rRNA gene regions as well as the study of the diversity of the rhizosphere microbiome by high-throughput sequencing of the variable regions of the 16S rRNA gene;
1.4 Screening and selection of strains according to their ability to nitrogen fixation, phosphorus mobilization and suppression of phytopathogens ((Fusarium oxysporum, Alternaria alternata, Pseudomonas syringae, Phytophtora cactorum, Cytospora carphosperma, Septoria piricola etc.)
- Compilation of consortia of microorganisms as the basis of biological preparations
- Study of the growth-stimulating activity of consortia of microorganisms on the seeds of annual and perennial plants, as well as seeds and seedlings of tree species used as afforestation material (apple (Malus), sea buckthorn (Hippophae), pear (Pyrus), birch (Betula), ash (Fraxinus)) in the laboratory and on the pilot sites of the Botanical Garden of Astana and the experimental garden of “F&V RI” LLP (www.fvri.kz).
- Optimize the medium and temperature regime for the cultivation of each consortium and accumulate their biomass.
- Calculate the optimal consumption rate of the preparations to suppress infection and calculate the most optimal treatment times.
- To study the effect of the preparations on the overwintering stock of infection during autumn processing and to study the effect of the drug on the quantity and quality of fruit crops. Analysis of data on the effectiveness of biological products in comparison with a chemical fungicide («Thiovit Jet» and / or «Horus» from Syngenta Crop Protection AG”; https://www.syngenta.kz )
- Conducting field trials of biological products in the forest nurseries of the Akmola region together with specialists of the Kazakh Research Institute of Forestry and Agroforestry, Shchuchinsk and “Zhasyl Aimak”, Astana and experimental garden of F&V RI LLP (www.fvri.kz).
- Submission of an application of intellectual property for the developed biological product to the National Institute of Intellectual Property of the Republic of Kazakhstan.
- Development of technological regulations and an act of implementation of the development.
Expected project results:
The result of the project will be biological preparations to stimulate the growth and survival of seedlings for afforestation and horticulture in the environment conditions of Kazakhstan. Field studies on the effectiveness of biological preparations will be carried out in the experimental garden of F&VRI LLP of the Ministry of Agriculture of the Republic of Kazakhstan, the botanical garden of Astana and forest nurseries, together with scientists in the field of plant protection and quarantine and forestry in Kazakhstan.
Name topic of the project: AP19678933 Genetic screening of newborns for immunodeficiencies in Kazakhstan: a pilot study
The purpose of the grant project: Carrying out genetic screening of newborns for immunodeficiency states based on the quantitative determination of circular DNA sections of T-cell and B-cell receptors (TREC and KREC).
Objectives of the grant project
Objective 1. Genetic screening of newborns: formation and replenishment of a biobank of DNA samples from the heel blood of newborns (at least 1000 samples) in the perinatal center No. 1 (Astana city). Carrying out genetic testing to determine the circular regions of DNA of the T-cell and B-cell receptors (TREC and KREC).
Objective 2. Creation and replenishment of an electronic database of demographic and clinical data of newborns included in the study.
Objective 3. Next-generation sequencing for DNA samples with a positive screening result, that is, with PID. Validation of NGS results by Sanger sequencing.
Objective 4. Bioinformatic analysis of the results of genetic screening of newborns.
Expected project results:
As a result of the project, data will be obtained from genetic screening of newborns for immunodeficiency conditions based on the quantitative determination of circular DNA regions of T-cell and B-cell receptors (TREC and KREC), and genes involved in the pathogenesis of PID will be identified. The results of the work will be reported at local and foreign conferences.
This project has a fundamental direction. Target consumers are medical and biological students, immunologists, oncologists, geneticists.
The proposed project is an interdisciplinary study where such fields of science as immunology, bioinformatics, and genetics intersect. The results obtained during the project in the future will be relevant for personalized medicine in the field of immunology, oncology, transplantology, selection of therapy and preventive measures.
This study is fundamental, but the results can be used in practical medicine in the form of guidelines and / or the introduction of genetic testing. Early diagnosis and successful treatment of diseases that are presymptomatic but potentially serious or fatal in infancy, thereby reducing preventable deaths and medical costs. Also, the results of NGS sequencing will improve the early diagnosis of congenital immunodeficiency states, which will contribute to timely diagnosis and the initiation of proper treatment. In general, this enables children with SCID to lead a healthy lifestyle.
The clinical and demographic picture of PID among newborns will be determined. Statistical data on the prevalence of PID will be obtained.